The cryo-EM method microcrystal electron diffraction (MicroED)
نویسندگان
چکیده
منابع مشابه
Atomic resolution structure determination by the cryo-EM method MicroED.
The electron cryo-microscopy (cryoEM) method MicroED has been rapidly developing. In this review we highlight some of the key steps in MicroED from crystal analysis to structure determination. We compare and contrast MicroED and the latest X-ray based diffraction method the X-ray free-electron laser (XFEL). Strengths and shortcomings of both MicroED and XFEL are discussed. Finally, all current ...
متن کاملHigh-Resolution Macromolecular Structure Determination by MicroED, a cryo-EM Method.
Microelectron diffraction (MicroED) is a new cryo-electron microscopy (cryo-EM) method capable of determining macromolecular structures at atomic resolution from vanishingly small 3D crystals. MicroED promises to solve atomic resolution structures from even the tiniest of crystals, less than a few hundred nanometers thick. MicroED complements frontier advances in crystallography and represents ...
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Incorporation of selenocysteine (Sec U) into proteins is directed by a in-frame UGA codon in all domains of life. In Bacteria, Sec biosynthesis and incorporation involves the interaction of Selenocysteine Synthase (SelA), tRNA (SelC or tRNA Sec ), Selenophosphate Synthetase (SPS), a specific elongation factor known as SelB and the specific mRNA structure SElenocysteine Insertion Sequence (SECIS...
متن کاملMicroED opens a new era for biological structure determination.
In 2013 we unveiled the cryo-electron microscopy (CryoEM) method of MicroED, or three-dimensional (3D) electron diffraction of microscopic crystals. Here tiny 3D crystals of biological material are used in an electron microscope for diffraction data collection under cryogenic conditions. The data is indexed, integrated, merged and scaled using standard X-ray crystallography software to determin...
متن کاملProtein structure determination by MicroED.
In this review we discuss the current advances relating to structure determination from protein microcrystals with special emphasis on the newly developed method called MicroED. This method uses a transmission electron cryo-microscope to collect electron diffraction data from extremely small 3-dimensional (3D) crystals. MicroED has been used to solve the 3D structure of the model protein lysozy...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2019
ISSN: 1548-7091,1548-7105
DOI: 10.1038/s41592-019-0395-x